5EU7
Crystal structure of HIV-1 integrase catalytic core in complex with Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2009-11-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.489, 87.650, 96.339 |
| Unit cell angles | 90.00, 95.83, 90.00 |
Refinement procedure
| Resolution | 95.840 - 2.640 |
| R-factor | 0.18401 |
| Rwork | 0.181 |
| R-free | 0.23317 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1EX4 and 1FVD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.624 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 95.840 | 2.729 |
| High resolution limit [Å] | 2.640 | 2.640 |
| Rmerge | 0.064 | 0.156 |
| Number of reflections | 42891 | |
| <I/σ(I)> | 11.54 | 5.92 |
| Completeness [%] | 98.7 | 92.99 |
| Redundancy | 7.53 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 20% PEG 3350, 100mM BisTrisPropane pH 7.0 |
