5EOA

Crystal structure of OPTN E50K mutant and TBK1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	291.15
Detector technology	CCD
Collection date	2015-07-25
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	36.824, 54.549, 153.305
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.444 - 2.503
R-factor	0.2018
Rwork	0.199
R-free	0.26680
RMSD bond length	0.007
RMSD bond angle	0.896
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.500
Number of reflections	11226
<I/σ(I)>	10.42
Completeness [%]	99.7
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6	291.5	0.1M MES monohydrate pH 6.0, 14% w/V Polyethylene glycol 4000