5ELV
Crystal structure of the GluA2 ligand-binding domain (S1S2J-L504-N775) in complex with glutamate and BPAM-521 at 1.92 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9753 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 98.790, 122.480, 47.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.801 - 1.920 |
| R-factor | 0.1644 |
| Rwork | 0.162 |
| R-free | 0.20920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tdj |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.011 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 34.250 | 31.801 | 2.020 |
| High resolution limit [Å] | 1.920 | 6.070 | 1.920 |
| Rmerge | 0.046 | 0.260 | |
| Rmeas | 0.097 | ||
| Rpim | 0.041 | 0.024 | 0.118 |
| Total number of observations | 228349 | 7047 | 32949 |
| Number of reflections | 44908 | ||
| <I/σ(I)> | 13.4 | 20.7 | 6.8 |
| Completeness [%] | 99.9 | 97.5 | 99.9 |
| Redundancy | 5.1 | 4.6 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 279 | 20% PEG4000, 0.3 M lithium sulfate and 0.1 M phosphate-citrate |
