5EGS
Human PRMT6 with bound fragment-type inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-22 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.918409 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 77.157, 135.616, 83.086 |
Unit cell angles | 90.00, 98.91, 90.00 |
Refinement procedure
Resolution | 41.260 - 2.150 |
R-factor | 0.20174 |
Rwork | 0.201 |
R-free | 0.24648 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4hc4 |
RMSD bond length | 0.021 |
RMSD bond angle | 2.134 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.145 | 0.775 |
Number of reflections | 90370 | |
<I/σ(I)> | 8.4 | 1.7 |
Completeness [%] | 99.2 | 96.8 |
Redundancy | 3.8 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 25 % PEG 1500; 0.1 M MMT pH 8.0 |