5EGO
HOXB13-MEIS1 heterodimer bound to methylated DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-25 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.984000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.976, 51.480, 113.575 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.544 - 2.540 |
| R-factor | 0.2296 |
| Rwork | 0.228 |
| R-free | 0.26480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xrm |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.065 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.630 |
| High resolution limit [Å] | 2.540 | 2.540 |
| Rmerge | 0.048 | 0.761 |
| Number of reflections | 8290 | |
| <I/σ(I)> | 15.5 | 1.6 |
| Completeness [%] | 98.7 | 98.8 |
| Redundancy | 4.2 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | PEG 5000, potassium chloride, magnesium chloride, Tris, PEG 400 |
