5EDM

Crystal structure of prothrombin deletion mutant residues 154-167 ( Form I )

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-08-14
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	109.884, 168.690, 144.315
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	92.070 - 2.200
R-factor	0.198
Rwork	0.196
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB CODE 4O03
RMSD bond length	0.012
RMSD bond angle	1.681
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	92.070	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.087	0.487
Number of reflections	67055
<I/σ(I)>	17	3
Completeness [%]	98.0	97.8
Redundancy	7.3	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1 M MES, pH 6.5 and 1.6 M MgSO4