5ED1
Human Adenosine Deaminase Acting on dsRNA (ADAR2) mutant E488Q bound to dsRNA sequence derived from S. cerevisiae BDF2 gene
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-24 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 82.362, 107.498, 121.097 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 80.392 - 2.770 |
R-factor | 0.1659 |
Rwork | 0.163 |
R-free | 0.22340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zy7 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.342 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX (1.9_1692) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.820 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.070 | 0.685 |
Number of reflections | 27727 | |
<I/σ(I)> | 11.57 | 1.52 |
Completeness [%] | 96.5 | 98.8 |
Redundancy | 2.93 | 3.01 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.1 M MES/NaOH, pH 6.5, 9% w/v PEG3350, 13% glycerol, 0.015 M NAD |