5E9J

Crystal structure of the mRNA cap guanine-N7 methyltransferase - modular lobe (416-456) deletion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-02
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.965
Spacegroup name	P 21 21 21
Unit cell lengths	70.760, 114.380, 134.810
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.806 - 3.470
R-factor	0.238
Rwork	0.236
R-free	0.26440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bgv
RMSD bond length	0.003
RMSD bond angle	0.784
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (dev_1702)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	87.210	3.710
High resolution limit [Å]	3.470	3.470
Rmerge	0.210	0.970
Number of reflections	13618
<I/σ(I)>	6.6	1.6
Completeness [%]	92.2	67.6
Redundancy	4.9	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	277	0.1M Hepes pH 7.8, 15% isopropanol and 6% PEG 4000