5E5A

Crystal structure of the chromatin-tethering domain of Human cytomegalovirus IE1 protein bound to the nucleosome core particle

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-06-25
Detector	PSI PILATUS 6M
Wavelength(s)	1.0308
Spacegroup name	P 21 21 21
Unit cell lengths	106.700, 109.474, 181.976
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.809
R-factor	0.1978
Rwork	0.196
R-free	0.24250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ID: 1AOI
RMSD bond length	0.008
RMSD bond angle	0.947
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.900
High resolution limit [Å]	2.800	6.020	2.800
Rmerge	0.133	0.076	0.611
Rmeas	0.144	0.083	0.664
Rpim	0.056	0.034	0.256
Total number of observations	346849
Number of reflections	52496
<I/σ(I)>	5
Completeness [%]	99.9	99.7	100
Redundancy	6.6	6.2	6.6
CC(1/2)		0.984	0.856

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	289	sodium cacodylate, magnesium acetate, 2-methyl-2,4-pentanediol