5E3R
Crystal structure of DapD in complex with 2-aminopimelate from Corynebacterium glutamicum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-15 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97934 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 92.446, 92.446, 279.602 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 93.200 - 1.850 |
| R-factor | 0.179 |
| Rwork | 0.178 |
| R-free | 0.20570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5e3p |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.132 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 93.200 | 1.830 |
| High resolution limit [Å] | 1.850 | 1.800 |
| Number of reflections | 36690 | |
| <I/σ(I)> | 32.36 | 4.15 |
| Completeness [%] | 97.8 | 97.8 |
| Redundancy | 5.1 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | PEG 200, MgCl2 |
