5E0U
Human PCNA variant (S228I) complexed with p21 at 1.9 Angstroms
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 3 |
| Unit cell lengths | 143.295, 143.295, 41.402 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.439 - 1.930 |
| R-factor | 0.1699 |
| Rwork | 0.162 |
| R-free | 0.22110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1axc |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.592 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.440 | 1.960 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.090 | 0.568 |
| Number of reflections | 71746 | |
| <I/σ(I)> | 20.7 | 2.96 |
| Completeness [%] | 99.8 | 98.65 |
| Redundancy | 7.7 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 100mM HEPES pH 7.5, 25% PEG 3350 |
