5E0K
X-ray crystal structure of tryptophan synthase complex from Pyrococcus furiosus at 2.76 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-25 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 87.862, 225.014, 296.241 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.910 - 2.760 |
| R-factor | 0.20594 |
| Rwork | 0.204 |
| R-free | 0.23774 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.172 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.810 |
| High resolution limit [Å] | 2.760 | 2.760 |
| Number of reflections | 150514 | |
| <I/σ(I)> | 8 | 0.6 |
| Completeness [%] | 99.3 | 93.7 |
| Redundancy | 6.7 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 297 | 16-20 % PEG3350 and 0.15-0.2 M Na citrate |
