5DY5
Crystal structure of human Sirt2 in complex with a SirReal probe fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-04-15 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 98.150, 53.360, 83.590 |
| Unit cell angles | 90.00, 114.01, 90.00 |
Refinement procedure
| Resolution | 45.850 - 1.950 |
| R-factor | 0.19066 |
| Rwork | 0.190 |
| R-free | 0.20495 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rmg |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.264 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.850 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.087 | 0.683 |
| Number of reflections | 28744 | |
| <I/σ(I)> | 10.9 | 1.9 |
| Completeness [%] | 97.3 | 91.6 |
| Redundancy | 4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 277 | 17.5 % (wt/v) PEG 3350, 0.1 M HEPES |
