5DTD

Crystal structure of Fis bound to 27bp DNA F1-8C (AAATTCGTTTGAATTTTGAGCGAATTT)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2011-03-04
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	43.280, 93.200, 154.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.609 - 2.642
R-factor	0.2197
Rwork	0.216
R-free	0.25190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3iv5
RMSD bond length	0.008
RMSD bond angle	1.102
Data scaling software	Aimless (0.5.14)
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	79.770	79.770	2.780
High resolution limit [Å]	2.640	8.350	2.640
Rmerge	0.049	0.020	0.583
Rpim	0.029	0.013	0.345
Total number of observations	71833	2249	9702
Number of reflections	18446
<I/σ(I)>	17.2	38.3	2.7
Completeness [%]	96.5	97.6	89.9
Redundancy	3.9	3.3	4
CC(1/2)	0.999	0.999	0.813

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	0.2 M Sodium citrate, 0.1 M TRIS-HCl pH 8.5, 36% PEG 400