5DO9

Structure of regulator of G protein signaling 8 (RGS8) in complex with AlF4-activated Galpha-q

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	2014-06-16
Detector	MAR scanner 300 mm plate
Wavelength(s)	1.0383
Spacegroup name	C 1 2 1
Unit cell lengths	173.946, 95.881, 112.898
Unit cell angles	90.00, 94.31, 90.00

Refinement procedure

Resolution	30.000 - 2.600
R-factor	0.1804
Rwork	0.178
R-free	0.22550
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.584
Data scaling software	SCALEPACK
Phasing software	PHASER (2.5.6)
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.640
High resolution limit [Å]	2.600	7.030	2.600
Rmerge	0.125	0.048	0.585
Rmeas	0.146	0.056	0.687
Rpim	0.075	0.030	0.358
Total number of observations	211145
Number of reflections	56869
<I/σ(I)>	7.6
Completeness [%]	99.8	99.4	97.6
Redundancy	3.7	3.4	3.6
CC(1/2)		0.996	0.715

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	277.15	Ammonium acetate, BisTris pH 5.5, PEG 8000