5DK4
Crystal structure analysis of Tryptophanyl-trna synthetase from Bacillus stearothermophilus in complex with indolmycin and Mg*ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-01 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 62.041, 62.041, 219.058 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.020 - 1.900 |
| R-factor | 0.1702 |
| Rwork | 0.169 |
| R-free | 0.18890 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.994 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 31.020 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 34849 |
| <I/σ(I)> | 10.7 |
| Completeness [%] | 100.0 |
| Redundancy | 15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | hepes, potassium citrate, magnesium chloride |
