5DK4

Crystal structure analysis of Tryptophanyl-trna synthetase from Bacillus stearothermophilus in complex with indolmycin and Mg*ATP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2012-02-01
Detector	MAR CCD 165 mm
Wavelength(s)	0.97949
Spacegroup name	P 43 21 2
Unit cell lengths	62.041, 62.041, 219.058
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.020 - 1.900
R-factor	0.1702
Rwork	0.169
R-free	0.18890
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.994
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	31.020
High resolution limit [Å]	1.900
Number of reflections	34849
<I/σ(I)>	10.7
Completeness [%]	100.0
Redundancy	15

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	298	hepes, potassium citrate, magnesium chloride