5DCZ
Tankyrase 2 complexed with a selective inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2012-07-11 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 66.599, 66.599, 114.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.580 - 2.230 |
| R-factor | 0.18125 |
| Rwork | 0.180 |
| R-free | 0.20176 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3p0p |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.023 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.600 | 2.350 |
| High resolution limit [Å] | 2.230 | 2.230 |
| Rmerge | 0.055 | 0.462 |
| Number of reflections | 13081 | |
| <I/σ(I)> | 25.6 | 4.3 |
| Completeness [%] | 99.4 | 99 |
| Redundancy | 8 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 295 | Crystals were obtained by hanging drop vapor diffusion at 22 deg in droplets consisting of 0.2 uL protein and 0.2 uL of 1.4-1.6 M Na/KPO4 pH 8.2 |
