5D7V
Crystal structure of PTK6 kinase domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-03-03 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54789 |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.718, 76.308, 87.271 |
| Unit cell angles | 81.46, 75.92, 74.93 |
Refinement procedure
| Resolution | 50.000 - 2.330 |
| R-factor | 0.2218 |
| Rwork | 0.219 |
| R-free | 0.28160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2f4j |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.175 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.410 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Rmerge | 0.113 | 0.443 |
| Number of reflections | 50742 | |
| <I/σ(I)> | 7.3 | 1.9 |
| Completeness [%] | 96.3 | 94.2 |
| Redundancy | 2.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.23M Diammonium Phosphate, 18% PEG 3350, 1M Lithium chloride |
