5D5A
In meso in situ serial X-ray crystallography structure of the Beta2-adrenergic receptor at 100 K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-18 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0322 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 107.000, 170.000, 40.500 |
| Unit cell angles | 90.00, 106.25, 90.00 |
Refinement procedure
| Resolution | 43.960 - 2.483 |
| R-factor | 0.2142 |
| Rwork | 0.212 |
| R-free | 0.26230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rh1 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.487 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1690) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.570 |
| High resolution limit [Å] | 2.483 | 2.483 |
| Number of reflections | 23086 | |
| <I/σ(I)> | 7.27 | 1.11 |
| Completeness [%] | 95.1 | 91 |
| Redundancy | 5.7 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 30-35 %(v/v) PEG 400, 0.1-0.2 M Na2SO4, 0.1 M bis-tris propane pH 6.5-7.0 and 5-7 %(v/v) 1,4-butanediol |
