5D1P
Archaeal ATP-dependent RNA ligase - form 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-08 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.5414 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.500, 115.304, 91.567 |
| Unit cell angles | 90.00, 104.55, 90.00 |
Refinement procedure
| Resolution | 29.365 - 2.199 |
| R-factor | 0.1937 |
| Rwork | 0.192 |
| R-free | 0.24130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.306 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.200 |
| Number of reflections | 45957 |
| <I/σ(I)> | 9.69 |
| Completeness [%] | 89.3 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M Tris-HCl (pH 8.5), 0.2 M Lithium Sulfate and 40 % PEG400 |
