5CX6

Crystal structure of Thosea asigna virus RNA-dependent RNA polymerase (RdRP) complexed with CDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	80
Detector technology	CCD
Collection date	2011-04-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.976
Spacegroup name	P 21 21 2
Unit cell lengths	133.700, 149.600, 100.110
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	66.850 - 2.100
R-factor	0.1861
Rwork	0.184
R-free	0.23180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xhi
RMSD bond length	0.007
RMSD bond angle	1.062
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.850	2.175
High resolution limit [Å]	2.100	2.100
Rmerge	0.115	0.422
Number of reflections	115494
<I/σ(I)>	5.64	1.8
Completeness [%]	98.3	98.6
Redundancy	3.1	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	PEG 8000, Lithium sulfate