5CWR
Crystal Structure of human DNA polymerase lambda L431A mutant in complex with a one nucleotide DNA gap and dCTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2014-09-27 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 93.003, 148.712, 86.414 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.123 - 2.500 |
| R-factor | 0.2035 |
| Rwork | 0.202 |
| R-free | 0.23350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mgi |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.738 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 5.380 | 2.500 |
| Rmerge | 0.091 | 0.055 | 0.320 |
| Rmeas | 0.100 | 0.061 | 0.354 |
| Rpim | 0.042 | 0.026 | 0.148 |
| Total number of observations | 228026 | ||
| Number of reflections | 41766 | ||
| <I/σ(I)> | 9.1 | ||
| Completeness [%] | 98.7 | 97 | 92.8 |
| Redundancy | 5.5 | 5.2 | 5.2 |
| CC(1/2) | 0.997 | 0.939 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.15 M Potassium Bromide, 30% Polyethylene Glycol Monomethyl ether 2000 |
