5CL1
Complex structure of Norrin with human Frizzled 4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 143.291, 150.589, 92.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.800 |
| R-factor | 0.2639 |
| Rwork | 0.262 |
| R-free | 0.31050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4my2 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.770 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.910 |
| High resolution limit [Å] | 3.800 | 8.690 | 3.800 |
| Rmerge | 0.264 | 0.090 | 0.923 |
| Rmeas | 0.280 | 0.097 | |
| Rpim | 0.117 | 0.035 | 0.526 |
| Total number of observations | 107966 | ||
| Number of reflections | 18303 | ||
| <I/σ(I)> | 3.5 | ||
| Completeness [%] | 90.5 | 99.9 | 65.2 |
| Redundancy | 5.9 | 7.4 | 3.3 |
| CC(1/2) | 0.997 | 0.923 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 200 mM ammonium sulfate, 100 mM sodium cacodylate trihydrate (pH 6.5), 15% w/v polyethylene glycol 8,000 |
