5CC1

S425G Glucocorticoid receptor DNA binding domain - (+)GRE complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-02-18
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00
Spacegroup name	C 1 2 1
Unit cell lengths	176.721, 39.122, 132.099
Unit cell angles	90.00, 101.10, 90.00

Refinement procedure

Resolution	32.407 - 2.302
R-factor	0.2464
Rwork	0.245
R-free	0.27910
RMSD bond length	0.010
RMSD bond angle	1.409
Data reduction software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.300
Number of reflections	36884
<I/σ(I)>	13.7
Completeness [%]	94.2
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	0.1 M HEPES, 20% PEG 8000, and 4% ethylene glycol