5C6W
anti-CXCL13 scFv - E10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 193 |
| Detector technology | PIXEL |
| Collection date | 2012-11-29 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.980, 80.360, 119.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.540 |
| R-factor | 0.1544 |
| Rwork | 0.154 |
| R-free | 0.16350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3juy |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.090 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.11.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.630 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Rmerge | 0.044 | 0.496 |
| Number of reflections | 98395 | |
| <I/σ(I)> | 26 | 3.4 |
| Completeness [%] | 98.8 | 92.2 |
| Redundancy | 8.8 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 100 mM Tris pH 8.5, 2000 mM (NH4)2SO4 |
