5C6H

Mcl-1 complexed with Mule

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	298
Detector technology	CCD
Collection date	2014-05-18
Detector	APEX II CCD
Wavelength(s)	0.987, 0.988, 1.0
Spacegroup name	P 1
Unit cell lengths	31.790, 114.240, 135.980
Unit cell angles	90.12, 92.32, 90.01

Refinement procedure

Resolution	35.451 - 2.050
R-factor	0.2974
Rwork	0.295
R-free	0.34560
RMSD bond length	0.003
RMSD bond angle	0.654
Data reduction software	XDS (IA32)
Phasing software	Coot (0.8.1.1)
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.451	2.750
High resolution limit [Å]	2.050	2.050
Number of reflections	114746
<I/σ(I)>	3.26
Completeness [%]	95.6
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	PEG3350, calcium chloride