5C69

Crystal Structure of Prefusion-stabilized RSV F variant PR-DM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-11
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792368
Spacegroup name	P 41 3 2
Unit cell lengths	167.820, 167.820, 167.820
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.540 - 2.300
R-factor	0.1821
Rwork	0.180
R-free	0.21670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mmr
RMSD bond length	0.005
RMSD bond angle	0.858
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.540	2.380
High resolution limit [Å]	2.300	2.300
Rmerge		1.554
Number of reflections	36440
<I/σ(I)>	14	1.8
Completeness [%]	100.0	100
Redundancy	11.6	12

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	9.5	293	1.24M K/Na tartrate, 0.2M LiSO4, 0.1M CHES pH 9.5