5C69

Crystal Structure of Prefusion-stabilized RSV F variant PR-DM

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Fusion glycoprotein F0,Fibritin	polymer	492	54443.0	1	UniProt (P03420) UniProt (Q38650) Pfam (PF00523)	Human respiratory syncytial virus A	Protein F
2	B, C, D, E, F... (A)	SULFATE ION	non-polymer		96.1	7	Chemie (SO4)
3	I, J (A)	2-[N-CYCLOHEXYLAMINO]ETHANE SULFONIC ACID	non-polymer		207.3	2	Chemie (NHE)
4	K, L, M, N, O (A)	CHLORIDE ION	non-polymer		35.5	5	Chemie (CL)
5	P (A)	water	water		18.0	173	Chemie (HOH)

Sequence modifications

A: 26 - 491 (UniProt: P03420)

PDB	External Database	Details
Ile 67	Asn 67	engineered mutation
Ala 102	Pro 102	conflict
-	Arg 108	deletion
-	Arg 109	deletion
-	Glu 110	deletion
-	Leu 111	deletion
-	Pro 112	deletion
-	Arg 113	deletion
-	Phe 114	deletion
-	Met 115	deletion
-	Asn 116	deletion
-	Tyr 117	deletion
-	Thr 118	deletion
-	Leu 119	deletion
-	Asn 120	deletion
-	Asn 121	deletion
-	Ala 122	deletion
-	Lys 123	deletion
-	Lys 124	deletion
-	Thr 125	deletion
-	Asn 126	deletion
-	Val 127	deletion
-	Thr 128	deletion
-	Leu 129	deletion
-	Ser 130	deletion
-	Lys 131	deletion
-	Lys 132	deletion
-	Arg 133	deletion
-	Lys 134	deletion
Pro 188	Ser 215	engineered mutation
Val 352	Ile 379	engineered mutation
Val 420	Met 447	engineered mutation
Ser 487	His 514	linker
Ala 488	Asn 515	linker
Ile 489	Val 516	linker
Gly 490	Asn 517	linker
Gly 491	Ala 518	linker

A: 492 - 517 (UniProt: Q38650)

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	54443.0
Non-Polymers*	Number of molecules	14
	Total formula weight	1264.3
All*	Total formula weight	55707.3

*Water molecules are not included.