5C5A
Crystal Structure of HDM2 in complex with Nutlin-3a
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-12 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.620, 43.630, 52.670 |
| Unit cell angles | 90.00, 90.04, 90.00 |
Refinement procedure
| Resolution | 41.620 - 1.146 |
| R-factor | 0.1537 |
| Rwork | 0.153 |
| R-free | 0.16830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hg7 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.593 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.620 | 1.187 |
| High resolution limit [Å] | 1.146 | 1.146 |
| Rmerge | 0.068 | |
| Number of reflections | 53751 | |
| <I/σ(I)> | 12.58 | |
| Completeness [%] | 79.1 | 12.95 |
| Redundancy | 2.9 | 1.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M Potassium iodide, 2.2M Ammonium Sulfate |
