5C3G
Crystal structure of Bcl-xl bound to BIM-MM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-01-31 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 106.220, 106.220, 92.760 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.769 - 2.450 |
| R-factor | 0.2268 |
| Rwork | 0.224 |
| R-free | 0.27350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fdl |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.691 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.990 | 2.690 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.044 | 0.872 |
| Number of reflections | 11587 | |
| <I/σ(I)> | 29.2 | 3.39 |
| Completeness [%] | 98.1 | 97 |
| Redundancy | 8.7 | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 12% Peg 1500, 0.1M Sodium Acetate pH 5.5, 2.5M NaCl, 1.5% MPD |
