5C12
Nuclease domain of the large terminase subunit gp2 of bacterial virus Sf6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-09-09 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.03316 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 132.645, 57.519, 46.598 |
| Unit cell angles | 90.00, 98.76, 90.00 |
Refinement procedure
| Resolution | 18.971 - 1.517 |
| R-factor | 0.1592 |
| Rwork | 0.158 |
| R-free | 0.18320 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.208 |
| Data scaling software | Aimless (0.1.29) |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.540 | 29.540 | 1.520 |
| High resolution limit [Å] | 1.490 | 8.180 | 1.490 |
| Rmerge | 0.095 | 0.062 | 2.350 |
| Rpim | 0.064 | 0.045 | 2.350 |
| Total number of observations | 172494 | 1051 | 249 |
| Number of reflections | 52497 | ||
| <I/σ(I)> | 9.6 | 22.1 | 0.1 |
| Completeness [%] | 93.1 | 95.1 | 8 |
| Redundancy | 3.3 | 3 | 1.1 |
| CC(1/2) | 0.989 | 0.985 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100mM HEPES, pH7.5, 50mM NaCl, 8% PEG8000 |
