5BV8
G1324S mutation in von Willebrand Factor A1 domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-12-17 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9774 |
Spacegroup name | P 61 |
Unit cell lengths | 86.450, 86.450, 68.160 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.230 - 1.590 |
R-factor | 0.164 |
Rwork | 0.163 |
R-free | 0.18100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4c29 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.021 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 43.230 |
High resolution limit [Å] | 1.590 |
Rmerge | 0.081 |
Number of reflections | 38781 |
<I/σ(I)> | 14 |
Completeness [%] | 100.0 |
Redundancy | 12.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 10.5 | 298 | 30% PEG 400, 100 mM CAPS/Sodium hydroxide |