5BTD
Crystal structure of a topoisomerase II complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-12-07 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.033 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 107.907, 83.158, 129.203 |
Unit cell angles | 90.00, 108.65, 90.00 |
Refinement procedure
Resolution | 47.394 - 2.497 |
R-factor | 0.2162 |
Rwork | 0.215 |
R-free | 0.24640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5bs8 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.741 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.394 | 2.586 | |
High resolution limit [Å] | 2.497 | 11.170 | 2.497 |
Rmerge | 0.137 | 0.019 | |
Rmeas | 0.158 | 0.027 | 1.727 |
Total number of observations | 285078 | ||
Number of reflections | 285133 | 1567 | 10299 |
<I/σ(I)> | 10.36 | 32.28 | 1.1 |
Completeness [%] | 99.7 | 93.4 | 97.56 |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 294 | 100 mM Tris-HCl, 13-15% PEG 4000, 200 mM MgCl2, 6.25% PEG 400 |