Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-08-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 211.210, 56.750, 65.450 |
| Unit cell angles | 90.00, 107.43, 90.00 |
Refinement procedure
| Resolution | 31.240 - 2.150 |
| R-factor | 0.224 |
| Rwork | 0.223 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gag |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.030 |
| Data reduction software | HKL |
| Data scaling software | SCALEPACK |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 43.351 | 43.351 | 2.130 |
| High resolution limit [Å] | 2.020 | 6.390 | 2.020 |
| Rmerge | 0.018 | 0.696 | |
| Rmeas | 0.044 | ||
| Rpim | 0.024 | ||
| Total number of observations | 163718 | ||
| Number of reflections | 48444 | ||
| <I/σ(I)> | 14.9 | 27.8 | 1.1 |
| Completeness [%] | 99.3 | 98.8 | 99.1 |
| Redundancy | 3.4 | 3.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | PEG 10000, (NH4)2SO4, BIS-TRIS |
