5AXW

Crystal structure of Staphylococcus aureus Cas9 in complex with sgRNA and target DNA (TTGGGT PAM)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-09
Detector	PSI PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	P 21 21 2
Unit cell lengths	67.596, 345.664, 98.052
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.670 - 2.700
R-factor	0.2046
Rwork	0.203
R-free	0.23030
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.597
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.670	2.770
High resolution limit [Å]	2.700	2.700
Rmerge		1.140
Number of reflections	64328
<I/σ(I)>	10.6	1.5
Completeness [%]	99.9	100
Redundancy	6.7	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG4000, NaCl, Na2HPO4, NaN3