5AWH

Rhodobacter sphaeroides Argonaute in complex with guide RNA/target DNA heteroduplex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-09
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.97921
Spacegroup name	P 1 21 1
Unit cell lengths	68.186, 118.303, 118.480
Unit cell angles	90.00, 95.83, 90.00

Refinement procedure

Resolution	35.630 - 2.000
R-factor	0.1866
Rwork	0.184
R-free	0.23250
Structure solution method	SAD
RMSD bond length	0.016
RMSD bond angle	1.758
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.030
High resolution limit [Å]	2.000	5.430	2.000
Rmerge	0.063	0.047	0.400
Rmeas	0.074
Rpim	0.039
Total number of observations	466156
Number of reflections	123866
<I/σ(I)>	15.3
Completeness [%]	97.7	74.6	100
Redundancy	3.8	3.4	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.05 M sodium cacodylate trihydrlate (pH 7.0), 42% (v/v) 2-methyl-2,4-pentanediol