5AOX

Human Alu RNA retrotransposition complex in the ribosome-stalling conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-03
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	81.480, 56.730, 170.590
Unit cell angles	90.00, 93.69, 90.00

Refinement procedure

Resolution	56.746 - 2.040
R-factor	0.1866
Rwork	0.185
R-free	0.22200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1E8O CHAINS A AND B
RMSD bond length	0.003
RMSD bond angle	0.723
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.800	2.090
High resolution limit [Å]	2.040	2.040
Number of reflections	49534
<I/σ(I)>	21.45	2.03
Completeness [%]	99.4	98.1
Redundancy	3.55	3.18

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.4		0.1M NA-MES,PH=6.4, 30%PEG400