5AGA
Crystal structure of the Helicase domain of human DNA polymerase theta in complex with AMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-02 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 116.170, 132.710, 156.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.632 - 2.900 |
| R-factor | 0.2198 |
| Rwork | 0.217 |
| R-free | 0.26440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.533 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.630 | 3.080 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.070 | 1.090 |
| Number of reflections | 26924 | |
| <I/σ(I)> | 22.8 | 1.9 |
| Completeness [%] | 99.3 | 98.5 |
| Redundancy | 5.6 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 19% PEG 3350, 0.2M POTASSIUM CITRATE TRIBASIC |
