5AEK
Crystal structure of the human SENP2 C548S in complex with the human SUMO1 K48M F66W
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 113.721, 119.319, 199.840 |
| Unit cell angles | 90.00, 89.67, 90.00 |
Refinement procedure
| Resolution | 47.000 - 3.000 |
| R-factor | 0.25935 |
| Rwork | 0.257 |
| R-free | 0.32625 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tgz |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.596 |
| Data reduction software | XPS |
| Data scaling software | CCP4I |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.760 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.160 | 0.460 |
| Number of reflections | 101157 | |
| <I/σ(I)> | 3.6 | 1.4 |
| Completeness [%] | 94.5 | 87.1 |
| Redundancy | 2.3 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 291 | 2M AMMONIUM SULFATE, 5% PEG 400, 0.1M BIS-TRIS PH 6.5 291K |
