5ACB
Crystal Structure of the Human Cdk12-Cyclink Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.780, 148.690, 91.580 |
| Unit cell angles | 90.00, 93.81, 90.00 |
Refinement procedure
| Resolution | 91.380 - 2.700 |
| R-factor | 0.22339 |
| Rwork | 0.221 |
| R-free | 0.26187 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.137 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 41.300 |
| High resolution limit [Å] | 2.700 |
| Rmerge | 0.070 |
| Number of reflections | 36020 |
| <I/σ(I)> | 12.5 |
| Completeness [%] | 98.7 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 10% PEG8000, 0.2M MAGNESIUM CHLORIDE, 0.1M HEPES PH7.0 |
