5A7R
Human poly(ADP-ribose) glycohydrolase in complex with synthetic dimeric ADP-ribose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-01 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.457, 91.277, 96.123 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 66.190 - 1.950 |
| R-factor | 0.13928 |
| Rwork | 0.137 |
| R-free | 0.17890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4a0d |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.547 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.220 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.100 | 0.580 |
| Number of reflections | 44881 | |
| <I/σ(I)> | 21.4 | 4.9 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 13.2 | 12.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 20% PEG3350, 0.2 M AMMONIUM SULFATE, 0.1 M PCTP PH 7.5 |
