4ZYO
Crystal Structure of Human Integral Membrane Stearoyl-CoA Desaturase with Substrate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 200 |
Detector technology | PIXEL |
Collection date | 2014-06-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.113 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 134.108, 134.108, 112.561 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.520 - 3.250 |
R-factor | 0.2424 |
Rwork | 0.241 |
R-free | 0.26360 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.941 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 116.000 |
High resolution limit [Å] | 3.250 |
Number of reflections | 18812 |
<I/σ(I)> | 19 |
Completeness [%] | 100.0 |
Redundancy | 20 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 100 mM Tris-HCl (pH 8.5), 30-35% (v/v) PEG400, 220 mM sodium citrate |