4ZVF

Crystal structure of GGDEF domain of the E. coli DosC - form II (GTP-alpha-S-bound)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-10-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97627
Spacegroup name	P 1 21 1
Unit cell lengths	28.500, 52.530, 50.930
Unit cell angles	90.00, 106.08, 90.00

Refinement procedure

Resolution	35.806 - 1.150
R-factor	0.1393
Rwork	0.138
R-free	0.16480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zve
RMSD bond length	0.006
RMSD bond angle	1.177
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.200
High resolution limit [Å]	1.150	1.150
Rmerge	0.042	0.416
Number of reflections	48048
<I/σ(I)>	13.07	2.14
Completeness [%]	93.6	72.8
Redundancy	3	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.1M imidazole pH 8.0, 0.2M calcium acetate, 22% (w/v) PEG 1000