4ZVF
Crystal structure of GGDEF domain of the E. coli DosC - form II (GTP-alpha-S-bound)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-10-04 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97627 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 28.500, 52.530, 50.930 |
Unit cell angles | 90.00, 106.08, 90.00 |
Refinement procedure
Resolution | 35.806 - 1.150 |
R-factor | 0.1393 |
Rwork | 0.138 |
R-free | 0.16480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zve |
RMSD bond length | 0.006 |
RMSD bond angle | 1.177 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.200 |
High resolution limit [Å] | 1.150 | 1.150 |
Rmerge | 0.042 | 0.416 |
Number of reflections | 48048 | |
<I/σ(I)> | 13.07 | 2.14 |
Completeness [%] | 93.6 | 72.8 |
Redundancy | 3 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 0.1M imidazole pH 8.0, 0.2M calcium acetate, 22% (w/v) PEG 1000 |