4ZRY
Crystal structure of the heterocomplex between coil 2B domains of human intermediate filament proteins keratin 1 (KRT1) and keratin 10 (KRT10)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.1 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 75.190, 75.860, 209.290 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.370 - 3.300 |
| R-factor | 0.273178625892 |
| Rwork | 0.273 |
| R-free | 0.27686 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tnu |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.609 |
| Data reduction software | XDS |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.370 | 3.380 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.073 | |
| Number of reflections | 9336 | |
| <I/σ(I)> | 13.76 | 0.5 |
| Completeness [%] | 99.6 | 99.4 |
| Redundancy | 6.4 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298.15 | 0.1 M HEPES, 2.0 M ammonium formate |
