4ZRJ

Structure of Merlin-FERM and CTD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-09-23
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	67.895, 68.202, 82.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.505 - 2.300
R-factor	0.1925
Rwork	0.186
R-free	0.24750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1isn
RMSD bond length	0.008
RMSD bond angle	1.097
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.370
High resolution limit [Å]	2.300	2.300
Rmerge		0.239
Number of reflections	17339
<I/σ(I)>	26.3	7.3
Completeness [%]	99.5	98.6
Redundancy	7.2	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	0.1 M Bis-Tris, 21% PEG 3000