4ZPR

Crystal Structure of the Heterodimeric HIF-1a:ARNT Complex with HRE DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-08-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97929
Spacegroup name	P 43
Unit cell lengths	66.735, 66.735, 243.644
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.845 - 3.902
R-factor	0.2721
Rwork	0.265
R-free	0.34100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zp4
RMSD bond length	0.005
RMSD bond angle	0.843
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.970
High resolution limit [Å]	3.900	10.550	3.900
Rmerge	0.045	0.028	0.790
Rmeas	0.052	0.034	0.918
Rpim	0.027	0.018	0.465
Total number of observations	36593
Number of reflections	9620
<I/σ(I)>	11.2
Completeness [%]	99.1	83.3	100
Redundancy	3.8	3.5	3.9
CC(1/2)		0.995	0.609

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	289	100 mM MES, pH 6.0, 100 mM calcium acetate, 15% PEG400