4ZPR
Crystal Structure of the Heterodimeric HIF-1a:ARNT Complex with HRE DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-08-26 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97929 |
Spacegroup name | P 43 |
Unit cell lengths | 66.735, 66.735, 243.644 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.845 - 3.902 |
R-factor | 0.2721 |
Rwork | 0.265 |
R-free | 0.34100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zp4 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.843 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.970 |
High resolution limit [Å] | 3.900 | 10.550 | 3.900 |
Rmerge | 0.045 | 0.028 | 0.790 |
Rmeas | 0.052 | 0.034 | 0.918 |
Rpim | 0.027 | 0.018 | 0.465 |
Total number of observations | 36593 | ||
Number of reflections | 9620 | ||
<I/σ(I)> | 11.2 | ||
Completeness [%] | 99.1 | 83.3 | 100 |
Redundancy | 3.8 | 3.5 | 3.9 |
CC(1/2) | 0.995 | 0.609 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 289 | 100 mM MES, pH 6.0, 100 mM calcium acetate, 15% PEG400 |