4ZPH
Crystal Structure of the Heterodimeric HIF-2a:ARNT Complex with Proflavine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 1 |
| Unit cell lengths | 48.896, 76.418, 98.345 |
| Unit cell angles | 89.83, 89.64, 73.21 |
Refinement procedure
| Resolution | 49.172 - 2.800 |
| R-factor | 0.189 |
| Rwork | 0.185 |
| R-free | 0.22060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zp4 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.525 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 7.590 | 2.800 |
| Rmerge | 0.054 | 0.026 | 0.693 |
| Rmeas | 0.069 | 0.033 | 0.908 |
| Rpim | 0.042 | 0.020 | 0.580 |
| Total number of observations | 79567 | ||
| Number of reflections | 33408 | ||
| <I/σ(I)> | 10.7 | ||
| Completeness [%] | 98.5 | 97.2 | 97.6 |
| Redundancy | 2.4 | 2.4 | 2.2 |
| CC(1/2) | 0.985 | 0.490 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 2% Tacsimate, pH 7.0, 6% PEG3350 |
