4ZMA
Crystal Structure of a FVIIa-Trypsin Chimera (ST) in Complex with Soluble Tissue Factor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-3 |
Synchrotron site | MAX II |
Beamline | I911-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-05 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.209, 80.898, 125.759 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.768 - 2.300 |
R-factor | 0.2429 |
Rwork | 0.240 |
R-free | 0.29470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | In-House FVIIa-WT:sTF-FFR similar to 1DAN |
RMSD bond length | 0.017 |
RMSD bond angle | 0.588 |
Data reduction software | XDS (March 30, 2013) |
Data scaling software | XSCALE (July 4, 2012 BUILT=20130706) |
Phasing software | PHASER (2.5.7) |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.770 | 2.382 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.157 | 1.920 |
Number of reflections | 29510 | |
<I/σ(I)> | 8.65 | 0.78 |
Completeness [%] | 96.0 | 87 |
Redundancy | 3.5 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.1 | 295 | 0.1M Cacodylate,11% PEG 8000 , with seeding |