4ZJV
crystal structure of EGFR kinase domain in complex with Mitogen-inducible gene 6 protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.459, 81.865, 96.190 |
| Unit cell angles | 90.00, 106.67, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.700 |
| R-factor | 0.18125 |
| Rwork | 0.179 |
| R-free | 0.23197 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rf9 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.173 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.110 | 0.600 |
| Number of reflections | 23122 | |
| <I/σ(I)> | 9.1 | 1.2 |
| Completeness [%] | 96.5 | 87.3 |
| Redundancy | 2.8 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.1 M Bis-Tris 0.2 M Ammonium acetate 25% PEG3350 |
