4ZIH
Crystal Structure of core/latch dimer of Bax in complex with BimBH3mini
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 95.730, 95.730, 37.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.680 - 2.500 |
| R-factor | 0.189 |
| Rwork | 0.186 |
| R-free | 0.23330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bd2 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.645 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.680 | 2.589 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.085 | 1.018 |
| Number of reflections | 6334 | |
| <I/σ(I)> | 18.46 | 2.09 |
| Completeness [%] | 100.0 | 99.84 |
| Redundancy | 7.7 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | Tri-sodium citrate, Sodium cacodylate |
